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The induction times were calculated based on scattered light values in the BioLector® system using previously determined OD600/scattered light correlation functions.
Patients were evaluated by the "equivalent METAVIR" scoring system, using previously established cut-off values.
The ten isolates used here for genome sequencing (Table 1) were selected to (i) represent different genera of dairy associated sporeforming bacteria; (ii) represent various phenotypic and metabolic characteristics (Table 2) and (iii) represent the predominant rpoB allelic types associated with the dairy production system, using previously reported data [ 5].
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Birchall is not against organic farming, and believes it possible that the problems he experienced in Kent were the result of the conventional system used previously, with its heavy reliance on herbicides.
DNA concentrations were determined applying the same system used previously for the gene expression experiment.
It is based on a published system used previously by a WHO-convened group [ 7].
The systems studied here are based on the two-strand system used previously to study free energy increments of RNA multibranch loops (13, 14).
In this portion of the study, disease severity was scored for the degree of synovitis using a linear scoring system used previously in our laboratory [ 11].
We included a pain monitoring system, used previously in people with patellofemoral pain syndrome [ 20], to help the patients adjust training with regard to their perceived pain.
The reactive-centre sequence was adopted in preference to the numbering system used previously [ 22] because the former contains functional information lacking in the latter.
For reasons of comparability, we employed the same model system used previously to knock down the brain acid soluble protein 1 (BASP1) encoding gene by transient siRNA nucleofection in the chicken DF-1 cell line.
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