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In the type II CRISPR system, transcript from the CRISPR array is first processed into small CRISPR RNA (crRNA).
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CDA and PW contributed extensive sequencing of the adult nervous system transcripts.
We have demonstrated maternal transfer of innate and adaptive immune system transcripts into Atlantic halibut embryos and their relation with future embryo developmental potential.
This contrasts with adenosine to inosine conversion (Bass 2002), which occurs mainly in noncoding human sequences and most targets are found in nervous system transcripts of animals (Rosenthal and Seeburg 2012).
Using a new Atlantic halibut 10 k custom oligonucleotide array, we have demonstrated maternal transfer of innate and adaptive immune system transcripts into Atlantic halibut embryos and profiled their expression in early developmental stages.
Among the genes associated with the endomembrane system, transcripts encoding three zinc transporters (ZIP1, ZIP4 and ZIP5) and a zinc inducer facilitator (ZIF1), which is involved in Zn homeostasis and Zn sequestration, were upregulated.
We also determined the numbers of individual EST clones in the raw data that showed high sequence overlap (90%and96%6% sequence identity; see additional file 3) as a way to gain some measure of the relative abundances of the putative immune system transcripts present in the EST libraries.
The database also contains curated information on T. aestivum transcription factors (2891 transcripts), E3 ubiquitin ligases of the ubiquitin-proteasome system (876 transcripts), hormone-responsive genes (467 transcripts) and seed storage proteins (55 transcripts; Figure 1).
A number of well-characterized genes were accurately delineated within our system of transcript filtration.
Numerous well-characterized genes with known expression patterns were accurately dissected as organ-enriched within our system of transcript filtration.
The "ground truth" for states is generated by employing a GMM-HMM baseline system to transcript the training data beforehand.
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