We employed the Pichia pastoris secretory expression system to express ARG I and the expression yield of it was as high as ~1510 mg/L.
In this study, we designed a robust prokaryotic expression system to express functional VHHs against HER2 receptor.
We used a baculovirus expression system to express fusion proteins of HCV core, RGD (Arg-Gly-Asp) peptide, and IFN-α2a fragments in Sf9 cells.
These data justify the usefulness of P. pastoris expression system to express HCV structural viral proteins which may be useful targets for HCV vaccine candidates.
Using an E. coli expression system to express a heterologous recombinant protein has several advantages; these include cost-effectiveness, ease of production, time-saving and others.
The final full-length gene was transformed into a prokaryotic expression system to express a large quantity of biologically active proteins.
Using a prokaryotic expression system to express heterologous recombinant protein has several advantages including time-savings, cost-effectiveness, ease of production, simplified characterization and others.
So far, only Quyen et al. [ 18] had chose a dual expression plasmid system to express an active lipase, but the dual expression plasmid was derived from pET22b and its construction process was complex.
Therefore, we switched to a Pichia system to express the anti-VEGF ccFv and scFv antibodies; the expression vector for ccFv is shown in Figure 1D.
We exploited this property of the Gal4 system to express a reporter gene dependent on PR activity, the expression of the reporter gene being inversely proportional to the autocatalytic activity of PR, thus serving as the basis for our assay.
We used a biolistic particle delivery system to express plasmids encoding GFP-tagged PTEN (to visualize PTEN expression) and DsRed (used as a cell marker) in individual neurons in organotypic slices of the hippocampus.
