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To address these potential issues in the MassCode system the specificity of the MC-PCR was validated, in the same tube, by performing a one-step multiplexed quasi-nested probe reaction.
In this experimental system, the specificity of the SLE autoantibodies was associated with the occurrence of antibodies against ribonucleoprotein in the SLE serum.
As in other diseases with a gene-for-gene system, the specificity of qualitative resistance to avirulent Z. tritici genotypes leads to selection for virulence (loss of avirulence).
The targeted DNA binding specificity is determined by TALE repeats through protein DNA contact in TALEN-mediated genetic modifications, whereas in the case of a CRISPR/Cas9 system, the specificity is determined by the gRNA binding through RNA DNA contact (Miller et al., 2011; Zhang et al., 2011; Jinek et al., 2012).
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In both of these systems, the specificity is inherently higher than with one gRNA (Cho et al., 2014; Esvelt et al., 2013; Ran et al., 2013a).
In the system validation, the specificity tests demonstrated that the probe was highly specific to only ATS (100% for LOQ range) in the tablets and could be used to quantitatively estimate the concentration of the ATS in the tablets.
The effect of the lack of organisation of projection neurons into discrete cortical laminae in this system on the specificity of connectivity is currently unknown, although it is noteworthy that the Reeler mouse cortex, in which cortical lamination is markedly disrupted, appears to function broadly normally (Simmons et al., 1982; Simmons and Pearlman, 1982, 1983).
However, we followed the manufacturer's immunohistochemical protocols and the P-CREB labelling distribution within the pain-producing areas of the trigeminal system supports the specificity of the antibodies used.
An advantage of the miRNA system is the specificity of RNAi, which should facilitate the simultaneous regulation of multiple outputs with different miRNAs.
A second one-dimensional system considers the specificity of a movement's beginning and end points to categorize motor skills (continuous motor skills vs. discrete motor skills).
The AAV-U7snRNA approach may therefore circumvent this T-cell activation and represents the best of both worlds, combining the efficacy of a viral gene transfer system with the specificity of the exon-skipping strategy.
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