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Arrays were analyzed in a ProteinChip system reader PCS-40000) using the ProteinChip Data Manager Software version 3.5 (Bio-Rad).
One used the Thermo Scientific Cellomics ArrayScan VTI HCS (high content analysis system) Reader and the Spot Detector Bioapplication protocol version 3, allowing analyses of >10,000 vesicles per sample, with a resolution of around 400 nm.
The reaction was stopped using 2 N sulfuric acid, and optical density reading was taken at 450 nm (ELISA reader: Organon Teknika Microwell system, Reader 230 s, Eppelheim, Germany).
Plasma samples were incubated with 0.2 mg/mL O-dianosidine and 158 μM hydrogen peroxide in 50 nM potassium phosphate buffer (pH = 7.0) at a ratio of 4 1, and changes in absorbance were detected at 410 nm using a microtiter photometric plate reader (Microwell System Reader 230 S; Organon Technica Corp., Durham, N. C).
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The company's point-of-sales system, readers, and business services reflect a coherent and effective design process.
It's happened with mobile phones, computers and even global positioning system readers.
(That's all they're asked — Shapiro said that in earlier versions of the system, readers offered more detailed feedback, but "it felt like homework" and "it quickly devolved into workshopping — you know, 'I wouldn't choose a semicolon here.'").
In addition to using the 5-point scoring system, readers interpreting the images can designate spectra as unusable if marked lipid contamination or misalignment of metabolite resonance peaks is present.
After 10 min of incubation at 68°C, samples were cooled on ice and absorbance was measured at 412 nm in a microplate reader (ETI-System reader; Sorin Biomedica Cardio S.p.A., Saluggia, Italy. The amount of methanol was estimated as described [ 80]. For uronic acid quantification, 4 mg saponified AIS samples were incubated in 200 μl of 2 M Trifluoracetic acid (TFA) at 121°C.
For more details on database selection for FVC systems, readers may refer to discussions in [9,13] and section 2.4.1 and section 2.11 in [47].
For further information on SLB-based assay systems, readers should refer to the excellent reviews by Sackmann and Tanaka [ 10], Groves [ 9], Groves and Boxer [ 77], Groves and Dustin [ 78], and Cooper [ 79].
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