Sentence examples for system previously established from inspiring English sources

Furthermore, we added MS2 sequences at the stem loops of single-guide RNA (sgRNA), and fused MS2 coat protein with CtIP, repurposing the system previously established for highly active transcriptional control, named synergistic activation mediator (SAM 12.

We therefore have adapted a cost-effective TALEN generation system previously established for mammalian cells for the use in P. tricornutum, allowing the assembly of TALENs in about two weeks.

Fixed kidney tissue was stained with hematoxylin and eosin for evaluation of glomerulosclerosis and tubular damage and cryopreserved sections of kidney tissue were stained with FITC conjugated antibodies to IgG, IgM, and C3 for assessment of immune complex deposition that was scored by a blinded reader using a scoring system previously established in our laboratory[30].

To determine the mean fecal consistency score, the owners evaluated and recorded throughout the study the consistency of each of their dog's stool according to a standardized fecal scoring system previously established by Moxham [ 14].

We adopted a promoter-mediated dominant selection system, previously established for the characterization of the cardiomyocyte transcriptome (Doss et al. 2007), in order to enrich for a population of AHR-positive cells.

In the present study, we assessed the direct effects of DEHP and its metabolites MEHP, 5OH-MEHP, 5OXO-MEHP, and 5CX-MEPP on testis development using an organotypic culture system previously established for the rat (Habert et al. 1991; Lassurguere et al. 2003).

Patients were evaluated by the "equivalent METAVIR" scoring system, using previously established cut-off values.

N. benthamiana was selected as a host system and previously established protocols were adapted to isolate and characterize PBs from leaves transformed with Zera-DsRed by agroinfiltration.

The combination of the Q system and the previously established GAL4 inducible system will allow the development of strategies for manipulating transgene expression in different cell types in the same fly.

For investigating the headache-relevant trigeminal system, we have previously established such a preparation for terminals in the dura mater of the hemisected rodent scull [9], and for the freshly dissected intact rodent trigeminal ganglion [10].

By combining such tagging system with our previously established method for purifying muNS inclusions from baculovirus-infected insect cells, we have developed a novel protein purification protocol.