These mRNAs were enriched for cellular functions related to protein glycosylation and membrane localization (Supplementary Data 6) and significantly overlapped with those identified with the Xrn1-AID system (p-value = 1.4e−29, Hypergeometric test).
A Kruskal Wallis test followed by false discovery rate correction was performed for total organogenesis events per nodulated root system (p-value of 0.16) and for total organogenesis events per transformed root system (p-value of 1.2e-05 1.2e-05
The GEA tool indicates that DCN protein interacting proteins regulate "collagen fibril organisation" (P value 3.66E-8 3.66E-8 morphogeneskin (P value 4.46E-06), "skeletal systemorphogenesis" (P value 8.02E-6), a series of annotations which suggest more detailed biological roles of DCN.
In total, 1659 genes from regions that transitioned from an active (promoter, enhancer, gene) state in EL to an inactive (repressed, heterochromatin, low signal) state in AH (Fig. 5C, top right) were strongly enriched for multiple developmental GO terms, including 'Respiratory system development' (P value 1.66 × 10−) (Fig. 5D).
Among the genes decreased by C/EBPδ removal, therefore normally activated, cell cycle and mitosis were at the top of the Biological Process list, with lipid biosynthesis, development (ectoderm, muscle, immune system) having p values lower than E-07.
If a system has a p value (Probabilities) of ≤0.05 it is accepted as a significant factor on the test result, as evidence indicates that the parameter is not zero; that is, the contribution of the proposed parameter has a highly significant influence on the measured response (Patel et al. 2004; Sonebi 2004a, b).
Pathway analysis of differentially expressed genes was performed using IPA (Ingenuity Systems), using a P value cutoff of <0.05 on differential expression and a log fold-change of at least 0.263, corresponding to a minimum expression change of 30% between young and old samples.
In this case, the aim is to define the value system (P).
Open image in new window Fig. 3 Measurement of Bohler's angle, the crucial angle of Gissane, and the calcaneal height Table 2 Fracture distribution according to the Sanders classification system Sanders classification Group A Group B p value Type II A 21 26 0.42 Type II B 68 56 0.36 Type II C 4 6 0.44 Type III AC 8 20 0.12 Type III AB 80 63 0.22 Type III BC 6 5 0.88 Type IV 15 15 0.68.
McNemar's test confirmed that performance difference between the systems is statistically significant (P value < 0.001).
The statistically significant overrepresentation of gene groups in functional categories relative to the whole genome was determined with the MIPS online FunCat system using hypergeometric distribution for P value calculation http://mips.gsf.de/proj/funcatDB/help_p-value.html.html
