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During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus®16 system kit and Power Plex®21 system kit.
The extracted DNA was amplified for 15 different genetic loci using a Promega PowerPlex 16 system kit (Promega).
cDNAs were obtained using Reverse Trascription System kit (Promega, Fitchburg, WI, USA) and random primers.
Adeno-X Expression System Kit was used for adenovirus production (BD Biosciences Clontech).
The transfections were carried out using the Profection Mammalia Transfection System Kit (PROMEGA).
The biotin-labeled cDNA probe was generated using the Ovation Biotin system kit (NeuGen).
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Sections were stained using a streptavidin-peroxidase system KIT-97200, UlTMasensitive TMaiXin MaiXin, China).
TUNEL analysis was performed using DeadEnd™ Fluorometric TUNEL System kit (Promega).
The luciferase activity was measured by the Dual-Luciferase Assay System kit (Promega) using a luminometer (model LB9501; Berthold Lumat).
Long Expand Template PCR System kit (Roche Diagnostics, Inc).
ELISA was performed using the BDNF Emax ImmunoAssay System kit (Promega), according to the manufacturer's protocol.
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