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In the process, the secondary integrity is verified.
Semantical integrity is verified by analyzing the number of instances of specific classes.
The in-house vector presented here was constructed by combining commercial pUC18 and the pDONR-221 plasmid's ccdB gene encoding region; toxic gene system sequence integrity was verified by sequencing, corresponding to the expected size (659 bp).
The RNA concentration and 260/280 ratio were determined by using NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE and RNAA integrity was verified by lab-on-chip technology using the Experion automated electrophoresis system Bio-Rad Laboratoriess, Madrid, Spain).
Construct integrity was verified by sequencing.
DNA integrity was verified by 2% agarose gel electrophoresis.
RNA integrity was verified on a 1.2% denaturing agarose gel.
RNA integrity was verified on non-denaturing agarose gels.
RNA was quantified and integrity was verified by agarose gel electrophoresis.
DNA integrity was verified by electrophoresis in a 1% agarose gel in TAE buffer.
RNA integrity was verified using Bioanalyzer (Agilent Technologies 2100 Bioanalyzer-Bio Sizing, Version A.02.12 SI292).
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