In recent years, the methylotrophic yeast Pichia pastoris has emerged as one of the most commonly used expression systems for membrane protein production.
High-throughput and highly sensitive detection systems for membrane proteins are now becoming a realistic goal.
These contact-method-based approaches can increase the flexibility in designing automated BLM-based systems for membrane protein assays.
Encouraged by the success with heterologous expression of GPCRs and channelrhodopsin ChR2, we set out to test the fly eye system also for membrane transporters.
The instability of the aptamer protein complex in a detergent system used for membrane protein solubilization and cell lysis adds an additional challenge to the identification of an aptamer's target.
(192) Free, immobilized, and supported liposomes have a long history of being used as surrogate systems for membranes.
The combination of physiological in vivo-like qualities of our membranes with robotic arraying has created a powerful system for studying membrane biology in a high throughput fashion.
To create a microanalysis system for studying membrane proteins, it is necessary to construct an efficient and easily useable platform to handle the plasma membrane or the lipid bilayer.
