Exact(15)
To repurpose this system for genome engineering, spacer sequences matching genomic loci of interest can be directly programmed into a heterologously expressed CRISPR array.
Here, we report the development and successful application of a multiplex CRISPR/Cas9 system for genome engineering of up to 5 different genomic loci in one transformation step in baker's yeast Saccharomyces cerevisiae.
Availability: MCFDR is implemented in the Genomic HyperBrowser (http://hyperbrowser.uio.no/mcfdr), a web-based system for genome analysis.
The detailed procedures described herein promise to accelerate the usage of CRISPR/Cas9 system for genome editing in sea urchin embryos.
We applied a CRISPR Cas9 system for genome editing of different industrial strains, and show simultaneous disruption of two alleles of a gene in several unrelated strains with the efficiency ranging between 65% and 78%.
Recently, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) system for genome modification has provided an additional tool for Xenopus researchers to achieve simple and efficient targeted mutagenesis.
Similar(45)
Here we describe a user-friendly microarray system for genome-wide analysis of gene expression in Tetrahymena.
In order to more fully exploit the Drosophila system for genome-wide screens, it is important to further characterize the replication block for VACV.
This study demonstrates the capability of the Sleeping Beauty T2/OncZ system for genome-wide insertional mutagenesis in somatic tissues in zebrafish and its potential for identifying novel cancer genes.
This study demonstrates the capability of the Sleeping Beauty T2/OncZ system for genome-wide insertional mutagenesis in somatic tissues in zebrafish and the potential for identifying novel cancer genes.
This limitation casts doubts on the suitability of the system for genome-wide expression analysis.
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