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In this system, each gene is classified in one or more groups, depending on its function.
In this microarray system, each gene is represented as 16 distinct pairs of 25-mer oligonucleotide probes.
For comparison to the cell-free system, each gene was then cloned into the pDEST17 vector for expression in E. coli and screened for the same activities (Table 2).
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Probes and primers were designed using Roche probe library system for each gene expression; 18S rRNA was used as reference.
In practice, we solve the above over determined system for each gene separately using least squares (implemented in the limma package), carrying forward only the gene-specific defeat effect for subsequent analyses.
This system frames each gene in an appropriate metabolic context and incorporates, beside pathway topologies (with enzymes as nodes and reactions as directed edges), a wide information on intervening compounds and the notion of essentialness that helps in filling gaps, genes that have not been detected but that must be present as a function for a pathway to be rigorously defined.
In this system, each viral gene is assigned a specific genotype based upon its nucleotide sequence and established percent identity cut-off values.
In this system, transcription of each gene segment is driven by bacteriophage T7 RNA polymerase, which can be supplied transiently by recombinant vaccinia virus (rDIs-T7pol) or by cells that constitutively express the enzyme.
In these cases, we use a binary naming system to describe each gene speciation event, adding an 'a' to one duplicate, and 'b' to the other.
The TAIR system uniquely identifies each gene model via an Arabidopsis Genome Initiative (AGI) locus code (e.g., AT5G58330) that identifies a region of transcribed DNA together with a suffix of the form.N, where N is a whole number.
The Arabidopsis community has developed a nomenclature system in which each gene is assigned a unique AGI (Arabidopsis Genome Initiative) locus identifier in a standardized format (e.g. AT5G46330).
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