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Calcium imaging was performed on free-moving worms by the imaging system as previously described (Li et al. 2012).
Spoligotyping was performed using the Luminex Multianalyte Profiling System as previously described [16].
Bone marrow chimeras were generated using the Ly5.1/5.2 reconstitution system, as previously described [6].
Chondrogenic differentiation was induced on cell pellet culture system as previously described [29].
Both lentiviruses are 3rd generation replication-incompetent lentivirus and were made using the four-plasmid system as previously described [2].
Adenoviral vectors expressing GFP, PGC-1α, or ERRα were generated using the AdEasy system as previously described [38].
Generation and titering of replication-defective ecotropic retroviral stocks was done with the kat packaging system as previously described [21].
The recombinant adenoviral vectors expressing various forms of ASYN were constructed using the AdEasy system as previously described [62].
Cellular sterols were extracted with hexane/methanol and analyzed on a reverse phase HPLC system as previously described [21].
SNPs failing MALDI-TOF genotyping were genotyped by a TaqMan® MGB biallelic discrimination system as previously described [14].
The severity of the gross pathological changes was scored using a semi-quantitative scoring system as previously described [15].
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