Sentence examples for system and sequence from inspiring English sources
Most of these approaches attack the difficult problem of what to do when relatively little is known about the regulatory system and sequence recognition by the protein.
Using a luciferase reporting system and sequence analysis, the potential target of miR-411-5p miR-411-5p miR-411-5pouty homolog 4 (SPRY4), wasch identifiedrotein kinase C α-mediated activation of mitogen-activated protein kinasproutyPKs), especially p38MAPK phomologylation.
Optimal read length for expression analysis is impacted by system and sequence characteristics [ 55] but the 46 bp read length was empirically determined to yield good sequence alignments to L. bicolor and aspen gene models.
SNP genotypes were obtained and critical SNPs were confirmed using a combination of ABI SNaPshot™ genotyping on an ABI377 DNA sequencer, Sequenom iPLEX SNP typing on a MassARRAY system, and sequencing on ABI3130xl and ABI 3730 platforms.
Kawazu was mainly responsible for the battle system and sequences, which he based heavily on the tabletop game Dungeons & Dragons and the RPG Wizardry.
Samples were purified using the Wizard SV Gel and PCR Clean-up System and sequenced.
Amplified PCR products were gel purified and cloned to pGEM®-T Easy vector system and sequenced subsequently.
HP73 was then subcloned into the pGEM-T easy vector with the use of the TA cloning system and sequenced to an automatic sequencing analysis [ 15, 16].
RT PCR products using primer pair 3028-3 anDMS4sqR2R2 (Table S1) were cloned using the pGEM-T Easy Vector system and sequenced.
CC282 was then subcloned into pGEM-T easy vector with the use of the TA cloning system and sequenced with the use of the Sequenase Version 2.0 DNA Sequencing System (United States Biochemical Co., Cleveland, OH).
The quantification and qualification of the cDNA library were assessed by an Agilent 2100 Bioanalyzer and ABI Step One Plus Real-Time PCR System, and sequenced on the Illumina HiSeq™ 2000 pair-end system (Illumina, USA).
