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It is expressed at greatest levels in the central nervous system, and expression is localized to a distinct area called the nucleus incertus (NI), situated in the caudoventral region of the pontine periventricular gray (6, 48).
Human MutS homolog 2 (MSH2), a crucial element of the highly conserved DNA mismatch repair system, and expression of MSH2 have been down-regulated by Hsp90 function inhibition in human lung cancer.
Quantitative reverse transcriptase PCR (RT-PCR) was performed using validated TaqMan Assays (Applied Biosystems) on an Applied Biosystems 7900HT PCR system and expression normalized relative to GAPDH as described [23].
Aquaporins are water selective channels with possible roles in the nervous system and expression levels were upregulated after brain injury.
In mice, L. paracasei F19 has been shown to affect the immune system and expression of genes involved in energy homeostasis and insulin sensitivity, and to regulate body fat storage and lipoprotein metabolism.
Toll-like receptors (TLRs) are important players of the innate immune system, and expression of the 10 TLRs known in humans depends on the cell subset and differentiation status.
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We thank Corina Bräuer, Eva Zatecka and Aniruddha Mitra for technical assistance, Regis Lemaitre and Aliona Bogdanova at the MPI-CBG Protein Expression Facility for providing the DefBac baculovirus expression system and compatible expression vectors, respectively, Hauke Drechsler for comments on the manuscript and Michael Thompson for language editing.
Production of these biologics with various plant systems and expression strategies is examined.
E. coli expression of Ab derived fragments has been highly successful and a number of vector systems and expression strategies exist ([ 21] and references herein).
These data also show that – unlike the melanocortin system and GAD65 expression – ARC NPY mRNA expression was not altered after PP injection.
"Under an authoritarian, one-party system, creativity and expression tend to be short-changed," Griswold says.
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