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To test this possibility, we studied the use of rhPCR to prevent primer-dimer formation in a Hepatitis C Virus (HCV) 1b V154 system (Accession #EU660388) where the need to position primers in specific areas of the viral sequence that are highly conserved between strains resulted in an assay extremely prone to the formation of primer-dimers [ 7].
Reverse transcriptase PCR (RT-PCR) was used for the amplification of the HA cleavage site sequence (from positions 451 to 1262 of the HA gene using sequence A/turkey/Ontario/7732/66 as numbering system; accession number AB558456), a marker for the virulence potential of AI viruses [ 15].
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Nevertheless, China is defending the collaborative, rules-based order because it has benefited hugely from the system: Its accession to the World Trade Organization in 2001 gave an immense boost to Chinese exports but also provided a real incentive for domestic economic reforms.
For the pYT08-codon-optimized EhMCF, a synthetic DNA encoding an EhMCF ORF in which the codon usage is optimized to that of the wheat germ translation system (GenBank accession number LC036596) was custom synthesized by GenScript Japan Tokyo, Japann) and cloned into SpeI and SalI sites of the pYT08 vector (12).
We needed to implement a strict scheme for system-generated accession identifiers in order to ensure unique, meaningful identifiers across all repositories.
A Chief Cataloger was appointed for the first time, to institute a modern system of accessions cataloging.
Its chemical shift assignments were also deposited with BMRB ADIT-NMR online deposition system under the accession number 36113.
The experimental vector is the mouse-specific SCOP/PHLPP1 open reading frame sequence in the Lv156 lentiviral plasmid system (Cat# Mm25576, Accession #NM_133831.3).
"Biological Process" contains immune system process (GO accession 0002736) attaching 11 subsets below (Table 10).
The normalized data were subjected to a Student t-test (P<0.01) and were required to have a median fold change of at least 2. The microarray data has been deposited in Gene Expression Omnibus (GEO) system under the accession number GSE12421.
The central object of the system is the accession in the sense of an anchor for the information belonging to the collection items.
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