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All experiments started after compensation for system aberration, therefore, all of the wavefronts presented here represent the corrections for sample-induced aberrations.
The principles of system aberration correction, wavefront sensing, image acquisition, control electronics and control software are the same as those used in our earlier work9 except that the two spatial light modulators working for VIS and NIR beams separately are now replaced by a single DM, which not only simplifies the system but also works over a wider wavelength range.
First, the system aberration was corrected with a model eye using a paper (scattering target) as a retina.
In the following experiments, the average system aberration (as measured on a thin test sample) was corrected before aberration maps measurements.
Note that during this measurement the deformable mirror of the AO retinal scanner was flattened and system aberration was not corrected.
For instance, there is temporal wavefront instability in the form of micro-fluctuations of accommodation [ 16, 30], tear films, a point scanning system aberration, involuntary eye motion, and some other factors.
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The calculation of the system aberrations is independent of the number of surfaces and their locations relative to a pupil plane, thus the aberration-based design principles apply to systems with any number of surfaces, including those with intermediate images33.
Again, system aberrations at the centre of the field of view were corrected on a test sample before measurements.
Because we put the mirror (reflector) as a sample, it is impossible to correct the system aberrations with this double pass configuration.
System aberrations were first corrected on a test sample and the remaining aberration maps measured on this test sample were subtracted from the maps measured on the brain slice.
While a large extent of the system aberrations can already be minimized during optical design and alignment, the variation in aberration structure and image quality of human eyes can be quite large [ 3].
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