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Using your forefinger of the hand holding the syringe, guide the needle into the center of one side the tent you formed with your other hand in the first preceding step.
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After reclining the neck muscles from the midline, CSF was collected with a syringe guided in the cisterna magna by way of a stereotaxic holder.
Custom syringe needles, guides, and seating rods were used to pierce the meniscus and fix the markers at a depth of 1 mm below the meniscal surface with cyanoacrylate.
Direct intracerebral administration of drugs or respective vehicle was conducted with a stainless steel cannula connected by a polyethylene tube to a SGE 1-microlitre 26-gauge syringe, inserted through the guide cannula and extended 1.5 mm beyond the tip of the guide cannula to reach the cerebral ventricle.
The plunger remains stationary with respect to the syringe as the positioning guide automatically retracts.
The venous return time was the time between the first penetration of the skin and aspiration of venous blood into the syringe, allowing insertion of the guide wire.
Microinjection cannulae (29 gauge) which extended 2.0 mm beyond the ventral tip of the guide, were attached to a syringe pump via PE-20 tubing and inserted into the guide cannulae.
The manual ruler guide with a 10 μl syringe and attached 26-gauge, 6-inches needle (Hamilton Company, Reno NV/Bonaduz, Switzerland) filled with the contrast solution and straightened by a guiding cannula was then placed in the instrument sleeve of the articulated arm.
Microinjections were made with a stainless steel injector cannula (29 gauge), extending 2.0 mm beyond the ventral tip of the guide, and attached to a syringe pump via PE-20 tubing.
Intracerebroventricular infusions were performed using a Hamilton syringe attached to PE 50 tubing and an internal guide cannula extending beyond the guide cannula into the lateral ventricle.
The positioning guide is stabilized relative to the patient as the syringe carrier, syringe, and cannula are withdrawn.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com