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This principle has been used in the design of a synthetic module for 2-ketoacid elongation.
All genes encoding pathway enzymes were expressed under the control of a synthetic module that included promoters, 5′-untranslated regions, and terminators as a re-organized single operon in the chromosome.
A mathematical description of the synthetic module was developed.
A mathematical description of the synthetic module was produced.
This synthetic module was designed to generate a positive feedback response.
TE2s are well described in association with Pks and NRPS modules in bacteria, fungi and plants, and serve to proofread the nascent product on the synthetic module [39].
Similar(47)
We expressed synthetic modules expressing genes of the PEP-PTS and non-PTS pathways in Escherichia coli K12 for comparison.
Helical rosette nanotubes (HRN) are obtained through an entropically driven self-assembly process of low-molecular-weight synthetic modules under physiological conditions.
Moreover, the performance of the synthetic modules and 'blocks' needed to assemble more complicated networks requires more complete characterization as a function of arbitrarily complex environmental inputs.
The proposed estimation method for measuring retroactivity, based on the gene expression noise, can serve as a practical method for characterizing interface conditions between two synthetic modules and eventually provide a step toward large-scale circuit design for synthetic biology.
Prioritizing an open source philosophy for synthetic biology gives a very different impression than when foregrounding how synthetic biologists are attempting to create synthetic modules to be plugged into living materials and to generally expand the scope for turning synthetic DNA into profitable types of products.
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