Sentence examples for synthetic guide from inspiring English sources

CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses.

We target double strand breaks to specific sites within the genome by injecting mRNA encoding the Cas9 endonuclease and in vitro transcribed synthetic guide RNA into Drosophila embryos.

The use of synthetic guide RNAs is also uniquely suited to genome-scale high throughput arrayed screening, particularly when using complex phenotypic assays for functional genomics studies.

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a newly developed tool for site-specific cleavage of DNA targets directed by a synthetic guide RNA (gRNA) base-paired to the target DNA sequence.

Finally, the use of synthetic guide RNAs along with DNA-free sources of Cas9 (mRNA or protein) allows for transient CRISPR-Cas9 presence in the cell, thereby resulting in a decreased probability of off-target events.

Among the known custom DNA-binding modules, the nuclease-dead Streptococcus pyogenes Cas9 (dCas9) protein, which recognizes a specific DNA target through base pairing between a synthetic guide RNA and DNA, outperforms zinc-finger proteins and transcription activator-like effectors, both of which target through protein DNA interactions1.

Synthetic guides directed to the first and second exons of Tbx3 were used to generate individual stable cell lines that were screened by genomic DNA analysis and protein expression by sequencing (data not shown) and western blot (Fig. 8e), respectively.

The present work is designed to evaluate the periodontal condition after immediate implant placement and the success rate of implant with autogenous versus synthetic guided bone regeneration.

This review highlights the recent advances in synthetic nerve guide designs for peripheral nerve regeneration, and the in vivo applicability and future prospects of these nerve guide conduits.

The synthetic chimeric guide RNAs (gRNAs) discriminate a single-nucleotide polymorphism (SNP) difference in rat embryonic fibroblasts, allowing allele-specific genome editing of the dominant phenotype in (F344 × DA F1 hybrid embryos.

A synthetic chimeric guide RNA (gRNA), consisting of a fusion between crRNA and trans-activating crRNA, has been shown to direct Cas9 cleavage of target DNAs that are complementary to the crRNA.