Exact(22)
Acetyl-ACP and malonyl-ACP react in a reaction catalyzed by β-ketoacyl-ACP synthetase so that the acetyl moiety (CH3CO−) is transferred to the malonyl moiety (-OOCH2CO−).
The activation step, catalyzed by an aminoacyl tRNA synthetase specific for a particular amino acid, effects the formation of an aminoacyl AMP complex [88a] in a manner somewhat analogous to reaction [77]; ATP is required, and inorganic pyrophosphate is a product.
The toxin produced by V. cholerae attaches to the intestinal cells, the enterocytes, where it stimulates the membrane enzyme adenylate cyclase; this in turn interferes with the intracellular enzyme 3′,5′-cyclic adenosine monophosphate synthetase (cyclic AMP), thereby disrupting the sodium pump system for movement of water and allowing potassium and bicarbonate to seep out of the cell.
When ADP and Pi are bound to ATP synthetase, the excess of protons (H+) that has formed outside of the mitochondria (an H+ gradient) moves back into the mitochondrion through the enzyme complex.
The amino acids are loaded onto the tRNAs by specialized enzymes called aminoacyl tRNA synthetases, usually with one synthetase for each amino acid.
Attached to the crista is a complex enzyme (ATP synthetase) that binds ATP, ADP, and Pi.
The precise mechanism by which the ATP synthetase complex converts the energy stored in the electrical H+ gradient to the chemical bond energy in ATP is not well understood.
Malonyl coenzyme A and a molecule of acetyl coenzyme A react (in bacteria) with the sulfhydryl group of a relatively small molecule known as acyl-carrier protein (ACP SH); in higher organisms ACP SH is part of a multienzyme complex called fatty acid synthetase.
Similar(3)
However, in some organisms, less than the full complement of 20 synthetases are required because some amino acids, such as glutamine and asparagine, can be synthesized on their respective tRNAs.
Carbon nitrogen (C−N) bonds are formed by the action of such enzymes as amide synthetases and peptide synthetases.
He cites the fact that certain genes, like those for the proteins known as helicases and amino acid synthetases, are duplicated in all three kingdoms, and that these duplications must have occurred in the common ancestor, before the kingdoms split.
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