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The peptides were amidated at the carboxy terminus by synthesizing on MBHA Rink Amide resin (EMD Novabiochem) as previously described.
We also varied probe length, synthesizing on the same chip oligonucleotides of 19, 21, 23, 25 and 27 nucleotides.
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Figure 3 LR-TEM image of one nanorod synthesized on the ZnO film prepared by PLD.
Oligonucleotides were synthesized on Applied Biosystem Synthesizer 391 on 1 μmol scale, using the standard phosphoramidite chemistry (Protocol I).
They were synthesized on 380A DNA Synthesizer (ABI, Foster City, CA).
Pyrene-containing oligonucleotides were synthesized on ABI 394 DNA synthesizer.
Peptides were synthesized on an automated multiple synthesizer (MultiSynTech, Germany) by standard Fmoc chemistry.
Oligoribonucleotides were synthesized on an automated MerMade synthesizer (BioAutomation, Irving, TX, United States) using standard phosphoramidite chemistry.
Oligonucleotides were synthesized on an ABI 394 DNA synthesizer.
Peptides were synthesized on an Applied Biosystems ABII) 433A peptide synthesizer.
Thymosin β4 was chemically synthesized on a model 431A peptide synthesizer using solid phase Fmoc chemistry.
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