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Oligonucleotides were synthesized on an Applied Biosystems 392 DNA/RNA synthesizer using the phosphoramidite method (17, 18), deprotected, and purified, as described previously (19, 20).
One of his best friends from their Cornell days, Aric Press, editor of The American Lawyer, said on Sunday: "In the last few months he communicated with a mouse and synthesizer, using the last strength in his right hand.
[11C]CH3I was produced within the same synthesizer using the dry method (gas phase conversion) described by Larsen et al. [22] with adopted modifications described by Kniess et al. [23].
Auditory recordings of 666 2- and 3-syllable nouns were generated with a text-to-speech synthesizer using the Nuance Speechify software with a female voice.
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Then, a sign synthesizer uses the IR information to generate the signs.
RNA samples were synthesized using the ABI-3400 Synthesizer with phosphoramidites including 5′-DMT-2′ TBDMS rG (N-iBu), rA (N-Bz), rC (N-Ac), rU (GenePharma, China), following the manufacturer's standard protocol.
FlpA peptides were synthesized using conventional fluorenylmethyloxycarbonyl chloride solid-phase peptide chemistry on an Applied Biosystems 431A Peptide Synthesizer using protocols supplied by the manufacturer (Applied Biosystems, Foster City, CA, USA) by the School of Molecular Biosciences Laboratory for Bioanalysis and Biotechnology at Washington State University (Pullman, WA, USA).
The two RNA strands containing 5′ monophosphates were synthesized using standard phosphoramidite chemistry as described above, with 5′ monophosphorylation performed on the synthesizer using Chemical Phosphorylation Reagent (Glen Research, Sterling, VA, United States).
This work presents our system based on genetic algorithm (GA) to automatically estimate the input parameters of the Klatt synthesizer using an analysis-by-synthesis process.
The oligonucleotide probes were synthesized in an Applied Biosystems DNA synthesizer using phosphoramidite chemistry.
What is lesser known is that that track contains a new instrument, the Moog electronic music synthesizer used by the Beatles on the album Abbey Road.
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