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In this study, 5′-dimethoxytrityl[N3]-2′-deoxycytidine-3′-[ 2-cyanoethyl - N, N-diisopropyl)]phosphoramidite was manually coupled to an Ac-dC-CPG solid support, followed by standard deprotection techniques performed on the DNA synthesizer (Scheme S2).
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PSi structures, PSi-Mg,h-NH2 (Mg = APTES, Mh = APDMES), were introduced in a suitable column reactor to be used in the automated synthesizer; the syntheses were performed according to the scheme reported in Figure 1.
The radioligand [18 F]AFM (2) was synthesized by the reported method (Scheme 1) [27] except using an automated synthesizer as previously described for the synthesis of 4-[18 F]-ADAM [25].
Thus, the target speech is synthesized using a STRAIGHT synthesizer.
They were synthesized on 380A DNA Synthesizer (ABI, Foster City, CA).
Fig. 1 Scheme of the commercial 11C-synthesizer used for the radiosynthesis and purification of [11C]erlotinib (SPE: solid phase extraction; PCV: product collection vial).
Pyrene-containing oligonucleotides were synthesized on ABI 394 DNA synthesizer.
Oligonucleotides were synthesized on an ABI 394 DNA synthesizer.
Oligonucleotides were synthesized on an Applied Biosystems Expedite 8909 DNA synthesizer.
The syntheses of 12 mer/13mer TO-PNA-peptide chimeras (Table 1) were carried out (Scheme 1) by Fmoc solid phase peptide synthesis on TG Sieber resin on a Protein Technologies PS3 synthesizer at the 10 μmol scale.
A synthesizer?
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