After the honeybees are infected by pathogens, four antimicrobial peptide families are synthesized, representing a broad spectrum of antimicrobial activity in the haemolymph.
Thus, from nine mock-inoculated and nine prion inoculated mice, a total of three sets of labelled cRNA probes were synthesized representing three biological replicates.
The primers designed for the mouse genome cover 27684 genes, but because of some redundancy – one primer pair can represent multiple genes, in most cases isoforms – only 26855 primer pairs were synthesized to represent once each of these 27684 genes (Table 2).
Nonribosomally synthesized peptides represent a large group of structurally complex metabolites that are manufactured from amino, hydroxy and carboxy acid monomers by large multifunctional enzymes, termed NRPS (Mootz and Marahiel 1997).
Thus, the energy content of the newly synthesized plumage represented just 6.9% (±0.57) of the energy expended for feather renewal.
Finally, a 24-base oligonucleotide (ss24-mer, GTCTGATTCATCTGCGTG-CTGACT) was synthesized to represent short single-stranded oligonucleotides.
Many of the conjugates synthesized here represent tRNA acceptor stem loop structures carrying an arginine-containing short peptide.
Six cDNA libraries from adult female mosquitoes were used to generate 8,020 Expressed Sequence Tag (EST) clusters [ 53] from which 60-mer oligonucleotides were synthesized that represent 6,143 unique EST clusters.
To resolve this discrepancy, we synthesized peptides representing heNOS CBD (residues 491 510) with point mutations at Thr site and determined the apparent affinity of the CBD variants to CaM.
Purine bases A(i and ii), 2- 6- benzyloxy -9 H-purin-9-yl)acetic acid (3A), and 2- 6- benzyloxy -9-9 H-purin-9-yl)acetic acid (3B), were synthesized as represented in Scheme 1 by reported methods.
