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Crosslinking treatments influenced the composition of newly synthesized matrix, and DHT treatment best matched the composition of native cartilage.
Overall, incorporating high-MW HA into degrading hydrogels increased chondrocyte number and sGAG and collagen production, warranting further investigations to improve retention of newly synthesized matrix molecules.
The success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix.
The increase in their dynamic modulus, |E⁎| (e.g., 2.4±0.4 kPa at 1 Hz to 9.7±0.2 kPa at 316 Hz at day 21, mean±SEM), and phase angle, δ, (e.g., 15±2° at 1 Hz to 74±1° at 316 Hz at day 21) with increasing frequency were attributed to the fluid flow induced poroelasticity, governed by both the newly synthesized matrix and the intracellular structures.
On days 0, 3 and 6 after exposure the rate of newly synthesized matrix macromolecules was measured.
Type 2 diabetes results in decrease of growth factors and cytokines required for the proper proliferative phase, increase in proinflammatory cytokines which result in spread of inflammatory phase, and increase in matrix degrading enzymes which disrupt the remodeling phase by degrading newly synthesized matrix and hence together bring impairment in wound healing [ 5, 6].
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To date, there are more than 20 BMPs members with diverse effects on the growth and differentiation of MSCs, as well as on their ability to synthesize matrix.
In atherogenesis, macrophages (MAC) and smooth muscle cells (SMC) are key actors; they synthesize matrix components and numerous factors involved in the process.
RCS cells were used in this set of experiments because unlike primary chondrocytes in monolayer that become fibroblastic and do not synthesize matrix proteins, RCS cells maintain a chondrocyte phenotype synthesizing COMP and other ECM proteins [29].
This finally results in the inhibition of many cellular processes that impair the capacity of the cells to synthesize matrix macromolecules and to repair damaged tissue [ 8, 31].
Notochordal-like cells have been shown to synthesize matrix in a manner distinct from mature NP cells (Cappello et al., 2006).
Related(19)
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