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For instance, in pharmaceutical companies, compounds are synthesized internally or purchased from external suppliers and can represent millions of individually identified chemical structures.
The ethyl-ester of the pan-caspase inhibitor Z-VAD-FMK was synthesized internally.
NVP-BSK805 (free base) was synthesized internally [ 14], 10 mM stock solutions were prepared in dimethyl sulfoxide (DMSO) and aliquots were stored at -20°C until use.
When this lipid is absent in the environment and not synthesized internally, the larvae remain in a state of arrested development, which can be overcome by resupplying the lipid.
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After 6 h, we observed the fluorescence of internally synthesized GFP by confocal microscopy (Fig. 2a).
The activities of internally synthesized GPAT and LPAAT were confirmed by detecting the produced lysophosphatidic acid and phosphatidic acid, respectively.
A more recent proposal, the exomembrane hypothesis, suggests that the nucleus instead originated from a single ancestral cell that evolved a second exterior cell membrane; the interior membrane enclosing the original cell then became the nuclear membrane and evolved increasingly elaborate pore structures for passage of internally synthesized cellular components such as ribosomal subunits.
The reported initiation mechanism of vRNA synthesis in this model describes that dinucleotide pppApG is internally synthesized de novo so as to be complementary to UC (in which the numbers indicate the nucleotide positions of the cRNA from the 3'-terminus and are denoted as 'c3'U4C5' in the discussion below).
The compounds were selected from commercial origin as well as from internally newly designed and synthesized chemical matter.
Internally labeled XBP1 mRNA transcript was synthesized in the presence of P-αGTP (Perkin Elmer).
Fluorescence pulse-chase analysis of an internally tagged Cse4 reveals that it is replaced with newly synthesized molecules in S phase, remaining stably associated with centromeres thereafter.
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