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After Dnase treatment (Sigma), first-strand cDNA synthesis was done using M-MLV RT (Promega).
Samples were either frozen at −80 °C until use, or cDNA synthesis was done directly.
The cost analysis of catalyst synthesis was done and found to be fairly economical.
cDNA synthesis was done using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with 500 ng or 1 µg input RNA depending on the sample group.
The synthesis was done in the presence of catalytic amount of KPS as an initiator and the adsorption behavior of the copolymer (360%G) was investigated by performing both the kinetics and equilibrium studies in batch conditions.
The synthesis was done by varying reaction time, concentration of monomer and initiator.
The synthesis was done by ion exchange and co-precipitation methods [31].
[11C]-acetate synthesis was done according to Pike [15] with minor in-house modifications.
Four different emulsifiers were used and the synthesis was done without any chain initiator or catalyst.
It is mentioned that the synthesis was done by passing nitrogen gas.
The whole synthesis was done in a heating mantle at 30 35 °C.
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