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The entire synthesis was completed on a single automated synthesis unit (GE Healthcare, Ecublens, Switzerland) over 124 132 min.
The Ms20-QD synthesis was completed by site-directed conjugation of Ms20 cys to maleimide-activated quantum dots through its carboxy-terminal cysteine.
Different feedstocks (citrus waste and microalgae) were selected and a conceptual process design from limonene oxidation to polymer synthesis was completed.
For RT-PCR analysis, first-strand cDNA synthesis was completed from total RNA (5 μg) with the SuperScript™ First-Strand Synthesis System for RT-PCR (Invitrogen Co., Carlsbad, CA, USA) and Oligo dT) primer.
First-strand cDNA synthesis was completed using a dedicated cDNA synthesis kit (Roche cat# 1117831), following manufacturers instructions.
A narrative synthesis was completed.
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Proteins lacking the ER signal sequence remain in the cytosol and are released from the ribosomes when their synthesis is completed.
In the subsequent S phase, DNA synthesis is completed to double the chromosomes for division.
The synthesis is completed using Mitsunobu azidation and peptide coupling as the key steps.
With this process, synthesis is completed in 14 min. Validation of the GMP-cassette synthesis is ongoing.
When the nanoparticle solution is collected in the chiller-cooled receiver, both the nanoparticle growth and the OR and OA processes continue to be suppressed by the cold environment and the inert surfaces, until the particle synthesis is completed.
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