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The gene encoding the G. graminis lipoxygenase AAK81882.1 was codon-optimized and synthesized by GenScript custom gene synthesis service (GenScript Corporation, Piscataway, USA) for expression in P. pastoris.
The oligo was made by custom primers synthesis service (Invitrogen).
The designed 1,629 bp double-strand cDNA was synthesized using a customized DNA synthesis service at TOYOBO (Osaka, Japan).
All peptides used in this study were ordered either from Mimotopes (Pensby, UK) or from the in-house peptide synthesis service at the Institute for Animal Health (Compton, UK).
Short amplicon DNA melting enables the creation of customized positive control samples for assays targeting rare alleles by simply making use of a commercial oligonucleotide or gene synthesis service.
The DNA fragments containing the mutations were synthesized by GeneArt Gene Synthesis service (Life Technologies, USA).
Similar(47)
Right now, synthesizers are too specialized for home use, but companies like DNA 2.0 offer synthesis services at reasonable cost.
To date, commercial DNA synthesis services are capable of synthesizing DNA in lengths up to tens of kilobase.
The questions were developed from the BIBS study evidence syntheses, service-user involvement, qualitative interviews and piloted for face validity with the target populations.
This paper describes an architecture-based approach for the synthesis of services from legacy components and their subsequent integration with service-requesting client applications.
Likewise, a meta-synthesis of services for women suffering from substance use issues identified that integrated services promoted recovery, personal growth and enhanced relationships with their children and significant others [ 17].
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