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Based on the minimal homology length of 35 bp and current oligo synthesis limit of 200 bp, it may be possible to knock in sequences of up to 130 bp using oligo templates.
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However, the relatively high cost for CNTs synthesis limits their large application in construction materials.
Moreover, the ultrahigh dilution conditions often required for single-chain polymer nanoparticle synthesis limits large-scale applicability.
A resource-constraint pipeline synthesis limits the area of a chip or the available number of functional units of each type.
This implies that the synthesis limits are very sensitive to the fuel equivalence ratio variation for the high stretch rate conditions, yielding a lot of impurities and soot rather than MWCNTs.
For peptides with lengths within the synthesis limits, chemical synthesis can deliver precisely designed molecules.
In turn, reduced prostaglandin synthesis limits inflammation and the development of hyperalgesic pain (Steinmeyer 2000).
Unfortunately, current experimental synthesis limits NIR absorbing SiO2/Au nanoshells to diameters above 100 nm.
Of the four bases, only dideoxy-C can be readily incorporated using standard methods of oligonucleotide synthesis, limiting widespread use of this technique.
In contrast, for trans-PtII anticancer complexes, monofunctional adducts and a small amount of 1,3-intrastrand cross-links are usually formed, 32 which may lower the level of DNA transcription and repair synthesis, limiting the development of resistance.
In addition, the possibility to generate single-strand probes in the range of 150 500 nucleotides by a sodium hydroxide treatment of immobilised probes with subsequent elution of the remaining biotinylated strand, opens up for new microarray applications that would extend probe length beyond current oligonucleotide synthesis limits.
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CEO of Professional Science Editing for Scientists @ prosciediting.com