The cDNA synthesis, labelling (biotin) and hybridization (Affymetrix GeneChip E.coli genome 2.0 array) were performed according to the Affymetrix GeneChip expression analysis protocols.
Additionally, with the longer half-life of the parental radionuclides sufficient time would be available to execute the synthesis, labelling and quality control in central radiopharmacies and distribute the products from there to the applying hospitals (Kim and Brechbiel, 2012).
Following mRNA isolation, cDNA synthesis, labeling, and hybridization were performed as previously described [80].
cDNA synthesis, labeling and purification were carried out using the Invitrogen cDNA labelling purification kit, while the Cy3 and Cy5 dyes were from GE Healthcare (Uppsala, Sweden).
Sample cRNA synthesis, labeling, hybridization and microarray processing were performed according to manufacturer's protocol "One-Color Microarray-Based Gene Expression Analysis" (version 1.0).
The cRNA synthesis, labeling and amplification were performed according to the user manual of the TrueLabeling-AMP™ Linear RNA amplification kit.
All cDNA synthesis, labeling, and hybridizations were performed using the Genisphere 3DNA Array 900MPX kit according to the manufacturer's protocol (Genisphere, Hatfield, PA).
cDNA synthesis, labeling and hybridization to Drosophila 385 k NimbleGen expression microarrays (Roche) was carried out as described in the manufacture's protocol with the exception that HS and RT samples were differentially labeled and hybridized to a single array.
NimbleGen performed: cDNA synthesis, labeling of the cDNA, and hybridization of the labeled cDNA to the Staphylococcus aureus COL custom chip (090327_Saur_COL_SACOL1232_Expr_X4 NimbleGen Systemss, Inc. Madison, WI) as per company protocols.
Synthesis, labelling and hybridization of cRNA to GeneChip® Yeast Genome 2.0 Arrays covering 5841 genes of S. cerevisiae was performed with RNA from 3 independent cultures of each strain following Affymetrix recommended protocols (http://www.affymetrix.com/analysis/index.affx).affx
