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In other words, the pitch synthesis error at the previous frame can be propagated to the next frame [12].
The minimization of the synthesis error function is also tackled with two different second-order methods; the best results are obtained with the use of both, each of them in different instants of the iterative process.
Hence, it should be possible to correctly assign samples with single base barcode sequencing errors, or to identify particular adapters with high rates of synthesis error [25].
To minimize the possibility of misidentifying samples as a result of sequencing or adapter synthesis error, all pair-wise combinations of barcodes differed by a minimum of three mutational steps.
This oligonucleotide synthesis error was exploited to determine the effect of the number of reads on within-sample variability; in essence the N-1 truncated tag allowed an examination of the technical replication of the experiment.
By combining these two steps, the CT synthesis error is decreased, on average, by 30%.
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However, directly applying these methods to depth maps may cause synthesis errors and lead to visual artifacts in the rendered virtual views.
The modifications of the method with extracts tailored for the detection of different kinds of DNA synthesis errors could be applied also as a simple approach to monitor replication fidelity in cell extracts.
A DNA fragment containing fur-wt, a downstream cat gene and flanking sequences (Fig. 3A) was PCR amplified in MnCl2-containing buffer to stimulate DNA synthesis errors, and the product was used to transform strain M1.5 Δfur-aphA, as described above.
DNA mismatch repair (MMR) proteins identify and correct DNA synthesis errors that occur during replication.
DNA synthesis errors can be introduced during two stages of the process: during selection of a correct dNTP for synthesis and at the extension step.
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