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Two independent cDNA syntheses were made from each RNA sample and duplicate QPCR reactions were performed for each cDNA to ensure reproducibility.
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Recently, the initial steps towards taking into account model's uncertainties under control synthesis were made in Haddad and Shraiber (2014) and Haddad (2015), where by considering one- and multi- region control problem, respectively, a robust perimeter control has been designed to systematically take into account uncertainties in MFD-based dynamics, e.g. the MFD scatter.
No measurements of the total amount of proteins in the cells or the overall rate of the protein synthesis were made in this experiment.
When pre vs. post clamp comparisons for variables such as glycolysis and glycogen synthesis were made, two- way ANOVA (group×time) was used.
Two technical replicates of cDNA synthesis were made from each RNA sample, both manually dissected and LCM.
This systematic review was conducted using best practice methods as recommended by the Cochrane Collaboration 6 13; important decisions on study selection, analyses, and data synthesis were made in advance of the searches being conducted.
Dp, delphinidin; Cy, cyanidin; Pt, petunidin; Pn, peonidin; Mv, malvidin; glc, glucoside Major steps forward in the study of plant anthocyanin synthesis were made through molecular physiology and forward genetics experiments [ 7].
The synthesis was made by analysing and synthesizing the key methodological and ethical elements in each study with the aim of transforming individual findings into new conceptualization and interpretations [ 32, 34].
The synthesis is made in MATLABs and MAPLEs computer systems.
cDNA synthesis was made of 2 μg of total RNA with "High-Capacity cDNA Reverse Transcription Kit" (Applied Biosystems, USA).
The controlled synthesis was made possible by the facile synthesis of pure PEG macroinitiators through a TEMPO-mediated oxidation.
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