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Upon α-factor washout, "WT" cells showed synchronous progression through S phase, metaphase, anaphase and telophase (Figure 4A).
FACS analysis determined S-phase progression (Fig. S2), while cell-budding demonstrated a synchronous progression through the cell cycle for all four strains (Fig. 2A).
Cells were arrested in early S phase by using a double thymidine block, and then released into fresh media to allow synchronous progression through the cell cycle.
Steroid-responsive breast cancer cells pretreated with a pure estrogen antagonist arrest in quiescence (i.e. G0) and respond to estrogen treatment with synchronous progression into the S phase.
In contrast to the proteins described above, protein levels of proliferating cell nuclear antigen (PCNA) remained constant during synchronous progression through the cell cycle.
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This allows synchronous meiotic progression as in Pankratz and Forsburg (Pankratz and Forsburg, 2005).
Thus, the expected synchronous cell cycle progression was achieved in pooled populations as first described for single cultures [23].
The first of these experiments (Fig. 3) examined the expression profile of C/EBPβ and cyclin A during the first 24 hrs of differentiation, which is known to include a phase of synchronous cell cycle progression.
Therefore, we validated six commonly used endogenous control genes under diverse experimental conditions of inflammatory stress, oxidative stress, synchronous cell cycle progression and cellular differentiation in 3T3-L1 adipocytes using TaqMan qRT-PCR.
Early stages of 3T3-L1 adifferentiationentiareon are characterized by synchronous cell cycle progression [17], [18], where quiescent preadipocytes cycle through one complete round of cell division within 24 hrs post-MDI concurrent with the onset of early stages of adipocyte gene expression [19], [20].
The partial hepatectomy model in rodents has been used extensively to investigate the mechanisms responsible for hepatic growth and proliferation, and is the best in vivo model of synchronous cell cycle progression in mammals [ 14, 15].
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