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These two scenarios also call for different public health actions: help to families with treatment difficulties, as has been an important part of the British Bug-Busting Program (16), or focus on frequent and synchronized checking routines followed by synchronized treatment, as has been the major focus so far in Norway (5, 6).
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On-going supervision includes biweekly Skype meetings between all local supervisors and the overall supervisors on the treatment delivery in order to discuss and synchronize treatment delivery across sites, and secure treatment manual adherence.
The cultures, before testing, were synchronized by treatment with 5% d-sorbitol with a parasitemia of 0.6 0.8%.
The parasite culture with a majority of rings was synchronized by treatment with 5% sorbital.
Prior to induction of gametocytogenesis, parasites were synchronized by treatment with 5% sorbitol at ring stage and cultivated at a parasitemia of about 1% under standard conditions.
Parasites were synchronized by treatment with 5% sorbitol at least 12 hours before sample collection.
Afer 6 d, fibroblast circadian rhythms were synchronized by treatment with 150 nM dexamethasone (Sigma-Aldrich) (65).
Malignant melanoma cells were synchronized by treatment with 100 ng/ml nocodazole for 16 h.
Bladder clocks were synchronized by treatment with 1 μM dexamethasone (27) for 1 h.
RPE-1 cells were synchronized by treatment with a CDK1 inhibitor, RO-3306, which blocks cells in G2 (see Figure 1A).
Briefly, 721 cells, HeLa-EBNA1, and 293 clones carrying EBV plasmids were synchronized by treatment with 15 μM aphidicolin (Sigma) or 100 ng/ml nocodazole (Sigma) for 16 h.
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