Exact(2)
We also synchronized primary human foreskin fibroblasts in G0 through a physiological process, i.e., contact inhibition, followed by release for various time points to allow progression through the cell cycle (Fig. 3B).
Second, the Bar-Joseph et al. dataset [ 35] of 17 profiles of synchronized primary human foreskin fibroblasts (FFs) for 0 32 h time points (2-h intervals) after release of double thymidine block and 2 profiles of serum starved FFs was investigated to identify genes which preferentially express in cycling cells.
Similar(58)
More precisely, the commit point is embedded in the transactional log and then is synchronized from the primary node to backups along with the log records.
This was first established robustly when Gray and Singer [13] showed that approximately 40-Hz oscillations were entrained and synchronized among cat primary visual cortical neurons that responded to the onset of a visual stimulus in their receptive fields.
The beta pattern was synchronized over the primary sensory cortex and subcortical areas.
Large sound reductions from these systems have been made possible through: (a) synthetically generating the cancelling sound and synchronizing with the primary source; (b) automatic alignment of all stability regions of the control system and; (c) avoiding instability produced by these multi-channel systems.
Primary fibroblasts we synchronized in G0/G1 by contact inhibition [34].
(A ) Primary HFFs were synchronized in G0 by serum deprivation (−FBS) and released (+FBS), and analyzed by Rb immunoblot, and Cdk4/6 and Cdk2 immunoprecipitation-kinase assays.
Primary keratinocytes were synchronized by a combination of contact inhibition and serum starvation for 24 h, after which they were released into complete growth medium containing 1 μM TPA.
To evaluate AURK levels in normal melanocytes and melanoma cell populations at the same point in the cell cycle, we synchronized melanoma cell lines and primary melanocytes by treating them with 100 ng/ml of nocodazole for 16 h, followed by mitotic shake-off, and performed Western blotting to analyse AURKA and AURKB protein levels.
Primary cortical neurons display synchronized calcium oscillations in culture, which were previously shown to be modulated by PTX, a GABAA receptor antagonist [ 44].
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