Then, cells were synchronized in the presence of serum-free medium for 24 h.
Ecdysone control of germline development, therefore, presents a positive feedback mechanism with which germline development in the germarium is synchronized with the presence or absence of older follicles.
In fact, our numerical simulations have verified that the order parameter would be invalid in describing synchronized switching in the presence of only extracellular noise and no intracellular noise, e.g., it is possible that R approaches to 1 in this case, but the synchronized switching are not achieved (data are not shown here).
That is, how its pacemaker cells try to synchronize in the presence of different light cues (like sunshine, artificial light or dim light from clouds) when arriving at various time zones three hours, six hours, nine hours and 12 hours away either to the east or west.
In this recording group, the presence of generalized activations synchronized across the recording sites is clearly observable (Fig. 8A,B).
All plasmid and siRNA transfections were performed for 48 to 72 h with cells synchronized for 24 h in the presence of 2 mM thymidine.
In this study, however, spatial acuity apparently became worse in the presence of simultaneously delivered synchronized sinusoidal stimuli – but only when they were applied to the unilateral stimulus sites.
60% confluent UTA6 cell cultures, in 20 large (15 cm) plates, grown in the presence of Tetracycline were synchronized using double-thymidine blocks and DMA treatment (14 h), and harvested by shake-off 24 h after Tetracycline release.
The most marked mitogenic effect was seen when the cells were fully synchronized by serum-starvation in the presence of ICI 182780 and subsequently stimulated by the addition of E2 (100-fold excess over ICI 182780).
Given the presence of skin sensor spiking stochasticity, synchronized spiking activity may well occur, as detailed next.
After 16 h of transfection, cells were synchronized at prometaphase, followed by release in the presence of CHX.
