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Cyclin B1 levels were shown to decrease sharply between 8 9 hours, as expected for a well synchronized population of cells, and it marks the transition through prophase, metaphase and into anaphase [69].
With this cell line, a highly synchronized population of cells is easily available by just shifting the culture temperature.
To obtain a highly synchronized population of G2 phase cells, we chose tsFT210 cells, which are temperature-sensitive Cdc2 mutant cells of mouse mammary carcinoma FM3A cells.
The approach requires a synchronized population of initiated transcription complexes containing RNA of a common length, which is achieved with promoters with a range of specific characteristics.
In each experiment approximately 10 15 worms were selected from a cultivation plate of a synchronized population of adults and rinsed with M9 buffer solution.
ChIP analysis on a sorted or synchronized population of early S-phase erythroid cells could address the question of whether this low level transcription is linked to transient histone modifications.
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We exposed synchronized populations of L4 nematodes to chloroquine and measured the release of mFP in different tissues.
Postembryonic phenotype characterization was performed by moving synchronized populations of L1 larvae to 26° for several (3 5) days and scoring adult germline morphology.
Our findings suggest 1) that high levels of synaptic background activity reduce the postsynaptic impact of poorly synchronized population signals, but permit the propagation of precisely timed, i.e. highly synchronized signals, and 2) that precisely timed spike patterns may be preferentially communicated via proximal synaptic terminals and between neurons with small membrane time constants.
We compared each population to the other two and failed to find a significant excess of genes that appeared to cycle in the evolved, synchronized population.
To reduce assay variability, a tightly-synchronized population of worms was selected based on size (i.e., stage of development) and fluorescence intensity (i.e., transgene expression) using the COPAS™ BIOSORT.
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