Recently, it has been published that, upon E2 induction, synchronized human cell lines exhibit a cyclical methylation/demethylation of the pS2 ERE region correlated with cyclical binding of transcriptional repressors/activators [47], [48], when the 5' end of pS2 is unmethylated [35], [48].
We describe an experimental system that was discovered by serendipity, which is characterized by a very fast, synchronized and complete induction of apoptosis.
To investigate VPCs induction and vulva morphogenesis, synchronized hermaphrodites carrying each transgene and the corresponding isogenic controls were heat-shocked in parallel at early L3 stage (33°C, 1 h, followed by 30°C, 1 h).
Prior to induction of gametocytogenesis, parasites were synchronized by treatment with 5% sorbitol at ring stage and cultivated at a parasitemia of about 1% under standard conditions.
Prior to induction of sexual commitment, asexual parasite cultures were synchronized for two cycles with 5% D-sorbitol [ 45].
Cells were counted in logarithmic phase (10 cells/mL, 2 mL in 6-well plates), which were synchronized by medium exposure, with a low concentration of FBS for 24 h for the induction of cell cycle arrest.
As (mathrm{dom}(m)) is well-behaved, so are also (mathrm{dom}(m_1)) and (mathrm{dom}(m_2)); therefore, by induction, we know that in the proofs of transitions (t_1) and (t_2) two sequences are never synchronized.
Soon their schedules were synchronized.
We are synchronized, now and forever.
