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We developed an accurate multiplex quantitative real-time PCR for synchronized determination of mtDNA and nuclear DNA (nDNA).
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For growth rate determination L. infantum promastigotes previously synchronized by 4 5 daily passages at 5×105 cells ml−1, were cultured at 106 cells ml−1 and the cell density monitored over time with a hematocytometer.
First, in contrary to unpredictable infections on the leaf, synchronized protoplast cells allows for a more precise determination of gene expression.
Numerical computation of the pair-wise phase interaction function outlined in Materials and Methods allows the determination of the stability of the fully synchronized (in-phase) solution.
Synchronized what?
Soon their schedules were synchronized.
"Everything in his life is synchronized perfectly.
SIGNATURE FEATURE Lights synchronized to the soundtrack.
Their deafening, synchronized roar dominated the dome.
We live in an increasingly synchronized world".
Baseball, football, tennis, synchronized swimming... whatever.
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