We tested eight different protocols in which either asynchronous cultures were transfected with siRNA, or cells were synchronized in combination with the siRNA transfection (Fig. 1B,C).
Parasites were synchronized using combinations of a magnet [41], plasmagel flotation [42] and/or Percoll gradient centrifugation to purify late-stage parasites [43], and sorbitol synchronization of ring-stage parasites as described previously [44].
Cells uniformly labeled with C-thymidine were synchronized by a combination of confluence arrest, followed by replating at lower cell density and treatment with aphidicolin for 24 hr.
Primary keratinocytes were synchronized by a combination of contact inhibition and serum starvation for 24 h, after which they were released into complete growth medium containing 1 μM TPA.
Randolph's Tube is a cylindrical metal structure lined with LEDs which, synchronized with sound, create incredible combinations of images and music that the artist intends to suggest the thrill of riding a wave.
The proposed system generates a dance for a humanoid robot through the combination of basic movements synchronized with the music.
In response to this need, a study was conducted to gauge the effectiveness of different multimedia combinations, namely: text; audio and synchronized text; audio, video and synchronized text, for procedural-based tasks to support web-based learning for a senior-level Production, Planning and Control Course.
Strikingly, we found that in the absence of noise epochs with highly synchronized activity were found for almost all combinations of gE and gI, whereas these seizure-like events were absent in simulations where noise was present.
Briefly, normal human fibroblasts (NHF1) obtained in this laboratory from neonatal foreskin [ 24] were synchronized to early in the S phase by a combination of confluence arrest, followed by replating at lower cell density in the presence of aphidicolin.
Dawood, Rieu, Wolters-Arts, Derksen, Mariani and Visser (2014) find that these root primordia commence synchronized growth to form roots within 2 3 days of flooding through a combination of cell division and elongation.
