Sentence examples for synchronized by at from inspiring English sources
For ADCI assays, blood stage parasite cultures were synchronized by at least two successive sorbitol treatments followed, after maturation over 24 h, by floatation on 1% porcine skin gelatin type A (Sigma).
The infection was synchronized by incubation at 4°C for 1 h to allow attachment and at 37°C for 1 h to allow virus entry.
Therefore, a cellular screening assay was developed, which focused on integrase activity, where infection of MT4 cells with an HIV-1 based lentiviral vector was synchronized by temporary arrest at the reverse transcriptase step and subsequent release to enable integration.
Subconfluent cultures of liver C9 cells were synchronized by metabolic arrest at the beginning of the S phase (see Fig. S1 in Supporting Information).
For growth rate determination L. infantum promastigotes previously synchronized by 4 5 daily passages at 5×105 cells ml−1, were cultured at 106 cells ml−1 and the cell density monitored over time with a hematocytometer.
All the grains were synchronized by imbibing with water at 4 °C for 24 h on sterilized CBD3.5 steel blue germination blotters (Ancho paper, St . PaulMN) in Petri dishes covered with aluminum foil and then placed in an incubator at 22 °C.
Cells were synchronized by serum-deprivation for at least 24 hours and stimulated with various concentrations of recombinant profilin (1 nM–10 µM) for 24 hours.
The dropping-RT-PCR was carried out on RNAs extracted from cells that were synchronized by double thymidine block at T0 (G1/S) and T+8 (G2/M).
ICQ values of the neurons were statistically analyzed using the one-sample t-test to compare the mean ICQ values against a random staining value of 0. Worms were synchronized by bleaching and harvested at the L4 stage (∼6,000 per sample) by rinsing worms off the plate using TE.
Cells were synchronized by serum starvation for at least 12 h before treatment.
Seeding of cells to the bottom of wells was synchronized by 1 min centrifugation at 1000 g.
