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In brief, synchronized ring-stage cultures (3%5%% parasitemia, 2.5% hematocrit) were treated with 5% sorbitol to lyse mature stages immediately after a 24-h exposure to 11 µmol/L artemisinin or 0.1% DMSO.
Video and laser vibrometer recordings were synchronized at the start by brief interruption of the laser path; this produces both a momentary peak in the oscillogram and a black frame in the video.
In brief, parasites were synchronized by 5% sorbitol lysis and diluted the following day into 75 cm flasks to reach 0.5% parasitemia at a hematocrit of 8.3% (total volume of 15 ml).
In brief, 5×10 cells synchronized at G0/G1 phase were lysed in lysis buffer [50 mM Hepes-NaOH (pH 7.5), 100 mM NaCl, 0.5% (v/v) Nonidet P40, 2.5 mM EDTA, 10% (v/v) glycerol, 1 mM and 1 mM PMSF].
One possible explanation is that the brief stimulus pulses elicit synchronized firing that binds or otherwise distinguishes the shape cues that are needed to access memory [ 4- 9].
Earle's fish-market debut required careful planning and perfect timing — the musicians did a brief walk-through the night before and synchronized their watches on the morning of the performance.
Seating should be able to be emptied and filled in less than 5 min during the brief break between all talks that is synchronized for all tracks.
Such seizures typically manifest themselves as brief periods in which neural activity is more synchronized than a certain baseline level.
In brief, it calls for the use of force to be clearly synchronized with political ends and for force to be applied decisively.
In brief, after transfection, cells were depleted of endogenous Bub1 by RNAi, synchronized with a double thymidine block and arrested in mitosis with nocodazole.
Synchronized what?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com