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In a further study, 119 mature cull ewes were inseminated following a 12-day synchronization treatment with frozen semen by either intrauterine (laparoscopic) or cervical insemination.
The effectiveness of the synchronization treatment to arrest cells in the M phase (0 h) was confirmed by both their 4C DNA content and by the expression of cyclin B1 (Figs. 6B and 6C respectively).
T4 was added 48 hours after the first synchronization treatment.
Whether this might influence the outcome of the synchronization treatment or not remains to be elucidated.
We also find that the periodical activity fluctuation of some TFs can be perturbed by the cell synchronization treatment.
This may be caused by noise or by the influence of synchronization treatment to the activity of transcription factors, which we will discuss in more detail in the next section.
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Rather, certain standard synchronization treatments cause damage that is the likely cause of G1 arrest.
Lactating dairy cows (n = 1,538) were enrolled in a completely randomized block design study to evaluate synchronization treatments.
Treatments designed to synchronize luteolysis, preovulatory follicular development, and ovulation, and resynchronize estrus after a first AI have improved responses to synchronization treatments.
No treatment differences were observed in either circulating concentrations of P4 or the ovulatory response to GnRH1 at the onset of synchronization treatments.
On Day 0 (start of synchronization treatments), heifers received a CIDR and either 100 μg GnRH i.m. (n=492) or 1 mg ECP plus 50 mg progesterone i.m. (n=491); in these groups, CIDR removal and PGF treatment were done concurrently on Days 7 and 8.5, respectively.
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