An efficient synchronization-release experiment circumvents the use of cell cycle markers and thus increases flexibility of imaging analysis.
SB-216763 and CT-99021 both attenuated CRMP4 phosphorylation following cellular synchronization and release with a double thymidine block or with nocodazole (Fig. 6B and 6C).
In contrast, BrdU-analyzed fields are consistently gathered from central positions on the plate, where the cells display similar monolayer densities, and comparable synchronization and release dynamics.
Following cellular synchronization and release, we did not observe any changes in threonine phosphorylation levels of CRMP1 (Fig. 6D) or CRMP2 phosphorylation at their GSK3 sites (Fig. 6E and 6F).
Because the mobility shift of Cdc24 on SDS-PAGE is maximal during bud emergence [12], [32] (Figure S1A and B), cell synchronization and release using cdc28-13, a temperature-sensitive allele of CDC28, was performed to enrich for small-budded cells, and thus phosphorylated Cdc24.
To address the differential impact of Cdk1 and Plk1 on centrosome separation, we compared the dynamics of centrosome separation following the activation of either Plk1 or Cdk1 using a series of synchronization and release experiments as described in Figure 2A.
Our algorithm avoids dedicated synchronization slots, releasing more time available for data transmission.
The first 8 hours following synchronization by thymidine release represent one complete cycle with >98% of the 2,833 cyclic genes showing a clear peak during this period.
The approximately 40% of cells remaining in G1 phase appeared irreversibly arrested in G1. p53 levels were assessed in cells during synchronization and after release from G2 phase.
Although the cues for the synchronization of gamete release in broadcast spawning corals are complex, our results indicate that nocturnal illumination is an important factor and that changes in the light regime surrounding corals can desynchronize the timing of gamete release.
A total of 29 Brahman and 24 Holstein cows were subjected to estrus synchronization using gonadotropin releasing hormone (GnRH) and prostaglandin F2α (PGF2α) superovulation.
