Predicted wavenumbers derived from B3LYP/6-31 B3LYP/6-31ethod synchronise well with those of the experimental observations.
Once that's done, the calendar, tasks etc should synchronise as well.
For one thing, it is easy to spot a crew that is not well synchronised.
"Warming by as little as 2°C (3.6°F) causes the males to emerge much earlier, meaning they are less well synchronised with the orchids," says Prof Davy.
Importantly, beta and non-beta cells revealed oscillatory profiles that were well synchronised with each other.
The oscillators are extremely well synchronised, with the mean field of the transmitter oscillating with a period of 24 h.
For these experiments we also increased the flow rate to 25 ml/min, to allow rapid (1.5 s) exchange of the tissue bath, and achievement of a relatively well synchronised Ca2+ release from the SR.
The clocks of dispersed beta and non-beta cells seem to be well synchronised among themselves, and they exhibit oscillations with a similar period length to that observed in a mixed population of islet cells.> We provide for the first time convincing evidence for cell-autonomous clocks displayed in cultured human islets.
A simulation of 10000 coupled oscillators similar to the total number of neurons in the SCN was also extremely well synchronised (data not shown), but for this study we concentrate on a more numerically tractable number of 200 oscillators for in depth analysis.
In order to estimate the 16 unknown parameters in this model, we defined a cost function (CF) that gave a low score for solutions that had a limit cycle oscillation of the mean field in constant conditions with a period of approximately 24 h, and were well synchronised (see Additional file 3, and [ 29] for details of the optimisation scheme).
