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Mycobacterial mechanisms involved in slow growth or switching growth rate provide rational targets for the development of new drugs against persistent mycobacterial infection.
Consistent with this, while EpiSCs require the stable exogenous expression of Klf4 or c-Myc for their conversion into conventional ESCs or, alternatively, the presence of small molecules that can replace these factors during reprogramming [4], [17], FGF-iPSCs can be converted to conventional LIF-dependent iPSCs by simply switching growth factor culture conditions.
Using chemostat culture to control growth rate, we screened a transposon mutant library by Transposon site hybridization (TraSH) selection to define the genetic requirements for slow and fast growth of Mycobacterium bovis (BCG) and for the requirements of switching growth rate.
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Controlled by critical heterochronic genes that switch growth on and off at different times and the genes that control the growth hormones that dictate the extent to which particular traits will develop, evolution of morphology and consequently, behavior, is very much determined by heterochrony.
C. albicans has a variety of properties that have been implicated in pathogenicity, including the ability to switch growth between a variety of morphological forms, such as yeast, pseudohyphal and hyphal forms.
The active region (AR) was deposited directly on the NW surface by switching the growth conditions from axial growth to radial growth [19, 21].
Microtubule dynamic instability can empirically be described by four parameters: rate of growth, rate of shrinkage, frequency of switching from growth to shrinkage (known as "catastrophe frequency") and frequency of switching from shrinkage back to growth (known as "rescue frequency").
Together, these results show that switching the growth factors could reverse the growth phenotype.
The critical pressure for switching the growth modes depends on reactivity of carbon feedstock to Cu substrate.
Twenty-four hours after transfection, the myoblasts were induced to differentiate by switching from growth medium (GM) to differentiation medium (DM).
To study the role of mATX3 in myogenic differentiation we transfected C2C12 myoblast cells with siRNA targeting the Mjd transcript, and induced their differentiation by switching from growth medium (GM) to a serum-limited differentiation medium (DM).
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