GFP mutants engineered with trypsin-sensitive sequences close to the eosin site, so that on trypsinolysis FRET was destroyed, the emission wavelength switching from that of the chemical FRET partner back to that of the native GFP fluorophore, providing efficient, ratio-based detection.
The earliest known Merry-Go-Round involved playing James Brown's "Give It Up or Turnit a Loose" (with its refrain, "Now clap your hands! Stomp your feet!"), then switching from that record's break into the break from a second record, "Bongo Rock" by The Incredible Bongo Band.
I even commiserated with her about how difficult it was to switch from that hierarchical world to being head of a college (I knew because my brother became master of Corpus, Cambridge, after retirement, and John Maud had made the same switch).
And a woman who identified herself as a singer asked Zander, "How do you switch from that sweet voice to those rawer vocals?" "Well, I have a switch right here on my back," Zander said, turning around and getting the evening's biggest laugh.
He was alone, as motionless as Inés on the beach, although now and then he switched from that drowsy, relaxed pose of head and elbow to another, apparently contrary position, his face buried in his hands, his feet tucked under him, as if he were exhausted or tense or possibly laughing to himself.
"My focus has not switched from that and it will not for a long time.
"Having said that we've just gone through a recession were there's been a lot of cost containment, so to switch from that to growth is going to be an interesting move".
The tension drains from the show when it switches from that commencement address to wheeling out the governor's office and having Taylor talk on the telephone to all and sundry while chatting with her offstage secretary (the voice of Julie White).
A crossover event was determined by noting the intervals in which the genotype of an offspring switched from that of one parent to that of the other.
Marie Hermetz, who paid Western Governors about $9,000 to earn her master's degree in health care management, said she heard about the program on the news and switched from one that would have cost up to $40,000.
The boring Australian I-told-you-so merchants have switched from blaring that the series will be too easy to blaring that Australia will always lose against spin.
