Sentence examples for switched templates from inspiring English sources

After MMLV-RT has switched templates to the DNA TS oligo, the process is repeated and TS oligo is added indeterminately to the 3' end of the growing cDNA strand (see Figures 1A/B and 3).

The two key features, template-switching and terminal transferase activity, of the enzyme can lead to adding a few non-templated C nucleotides to the cDNA and switching templates to transcribe the other strand [55] (Fig. 3b).

Indeed, RT polymerases are required to switch templates during normal retroviral replication, as part of the first and second transfer steps [48].

However, the frequency by which either base pair appears on CCC DNA also depends on the mechanism for plus strand DNA synthesis, which requires that nascent plus strands switch templates from r5 to r3 (Figure 1A).

RT then switches templates and continues replicating to the end of the oligonucleotide.

This phenomenon occurs during postcapture PCR library amplification, wherein a nascent DNA strand switches templates from one allele to another.

This model suggested that RT switches templates when it encounters palindrome (hairpin) structures that can induce RT to pause.

In contrast, Bst DNA polymerase can switch templates and is therefore less affected by the proximity of genes to the telomere [ 14].

The SMART™ II A Oligonucleotide base-pairs with the oligo(G) sequence and RT then switches templates and continues replicating to the end of the oligonucleotide [ 25].

Step 2 - The rG3 bases at the 3' end of a standard TS oligo anneal to the polyC bases left by the RT at the 3' end of the cDNA strand and RT switches templates, transcribing through the TS oligo DNA template.

In in vitro assays, L1 RT has demonstrated a high processivity on both RNA and ssDNA templates and the ability to switch templates from RNA to cDNA in order to synthesize the second strand cDNA [ 124, 137].